Spanel-Borowski, Katharina, Smith, Mark A., Simone, Cristiano, Simonsen, Anne, Simon, Hans-Uwe, ...
Research in autophagy continues to accelerate, and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring...
Net degradation of cellular components occurs in plant cells cultured under starvation conditions, and autophagy contributes to the degradation of intracellular proteins. In this study, we...
Net degradation of cellular components occurs in plant cells cultured under starvation conditions, and autophagy contributes to the degradation of intracellular proteins. In this study, we...
Inoue, Yuko, Suzuki, Takao, Hattori, Masaki, Yoshimoto, Kohki, Ohsumi, Yoshinori, Moriyasu, Yuji
In Arabidopsis root tips cultured in medium containing sufficient nutrients and the membrane-permeable protease inhibitor E-64d, parts of the cytoplasm accumulated in the vacuoles of the cells from...
Inoue, Yuko, Suzuki, Takao, Hattori, Masaki, Yoshimoto, Kohki, Ohsumi, Yoshinori, Moriyasu, Yuji
In Arabidopsis root tips cultured in medium containing sufficient nutrients and the membrane-permeable protease inhibitor E-64d, parts of the cytoplasm accumulated in the vacuoles of the cells from...
Yano, Kanako, Matsui, Sumiko, Tsuchiya, Tomohiro, Maeshima, Masayoshi, Kutsuna, Natsumaro, Hasezawa, Seiichiro, ...
Autolysosomes accumulate in tobacco cells cultured under sucrose starvation conditions in the presence of a cysteine protease inhibitor. We characterized these plant autolysosomes using fluorescent...
Takatsuka, Chihiro, Inoue, Yuko, Matsuoka, Ken, Moriyasu, Yuji
Tobacco (Nicotiana tabacum) culture cells perform autophagy and degrade cellular proteins in response to sucrose starvation. When protein degradation is blocked by the cysteine protease inhibitor...
Moriyasu, Yuji, Hattori, Masaki, Jauh, Guang-Yuh, Rogers, John C.
Autophagy in plant cells is induced by nutrient starvation. Initially, double membrane-bound organelles, termed autophagosomes, enclose a portion of cytoplasm, and then fuse with a vacuole or...
Isolation of the Red-light-absorbing Form of Phytochrome from Light-grown Pea Shoots (1981)
Shimazaki, Yukio, Moriyasu, Yuji, Pratt, Lee H., Furuya, Masaki
Phytochrome was extracted from both light-grown and dark-grown shoots of Pisum and partially purified by brushite chromatography and ammonium sulfate fractionation. About 160–270 ng of phytochrome...
Degradation of Proteins Artificially Introduced into Vacuoles of Chara australis1
Moriyasu, Yuji, Tazawa, Masashi
When an exogenous protein, bovine serum albumin, was introduced into the vacuole of a Chara australis internodal cell, it was degraded with time. This degradation proceeded only in the vacuole as far...
Partial Purification and Characterization of Aminopeptidase II from Chara australis
Moriyasu, Yuji, Miyoshi, Yasuhiro
Aminopeptidase II, one of the two major aminopeptidases in the giant alga Chara australis, was partially purified. Its molecular weight was estimated to be about 80,000 by gel permeation...
Moriyasu, Yuji, Sakano, Katsuhiro, Tazawa, Masashi
The presence of two major aminopeptidases (aminopeptidases I and II) in the giant alga Chara australis was shown using polyacrylamide gel electrophoresis. Partially purified aminopeptidase I had a...
Degradation of Proteins Artificially Introduced into Vacuoles of Chara australis1
Moriyasu, Yuji, Tazawa, Masashi
When an exogenous protein, bovine serum albumin, was introduced into the vacuole of a Chara australis internodal cell, it was degraded with time. This degradation proceeded only in the vacuole as far...
Partial Purification and Characterization of Aminopeptidase II from Chara australis
Moriyasu, Yuji, Miyoshi, Yasuhiro
Aminopeptidase II, one of the two major aminopeptidases in the giant alga Chara australis, was partially purified. Its molecular weight was estimated to be about 80,000 by gel permeation...
Moriyasu, Yuji, Sakano, Katsuhiro, Tazawa, Masashi
The presence of two major aminopeptidases (aminopeptidases I and II) in the giant alga Chara australis was shown using polyacrylamide gel electrophoresis. Partially purified aminopeptidase I had a...