Scientific Commons: Yukako Fujinaga

Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolated in Okayama Prefecture using pulsed field gel electrophoresis and random amplification of polymorphic DNA. (2006)

Funamori, Yuka, Fujinaga, Yukako, Yokota, Kenji, Inoue, Kaoru, Hirai, Yoshikazu, Oguma, Keiji, ...

Three outbreaks and many isolated cases of enterohemorrhagic Escherichia coli O157:H7 occurred in 1996 and 1997 in Okayama Prefecture, Japan. In an attempt to investigate the route of these...

Transport of Bacterial Toxins into Target Cells: Pathways Followed by Cholera Toxin and Botulinum Progenitor Toxin (2006)

Fujinaga, Yukako

A number of bacterial toxins have sophisticated mechanisms for reaching their specific targets in mammalian cells, to exert their toxicity. This review focuses on the transport mechanisms of cholera...

Isolation of a cDNA encoding the adenovirus E1A enhancer binding protein: a new human member of the ets oncogene family (1993)

Higashino, Fumihiro, Yoshida, Koichi, Fujinaga, Yukako, Kamio, Koichi, Fujinaga, Kei

The cDNA encoding adenovirus E1A enhancer-binding protein E1A-F was isolated by screening a HeLa cell λgt11 expression library for E1A-F site-specific DNA binding. One cDNA clone produced...

Purification of Fully Activated Clostridium botulinum Serotype B Toxin for Treatment of Patients with Dystonia

Arimitsu, Hideyuki, Inoue, Kaoru, Sakaguchi, Yoshihiko, Lee, Jaechul, Fujinaga, Yukako, Watanabe, Toshihiro, ...

Clostridium botulinum serotype B toxins 12S and 16S were separated by using a beta-lactose gel column at pH 6.0; toxin 12S passed through the column, whereas toxin 16S bound to the column and eluted...

Gangliosides That Associate with Lipid Rafts Mediate Transport of Cholera and Related Toxins from the Plasma Membrane to Endoplasmic Reticulm

Fujinaga, Yukako, Wolf, Anne A., Rodighiero, Chiara, Wheeler, Heidi, Tsai, Billy, Allen, Larry, ...

Cholera toxin (CT) travels from the plasma membrane of intestinal cells to the endoplasmic reticulum (ER) where a portion of the A-subunit, the A1 chain, crosses the membrane into the cytosol to...

Cholera Toxin Toxicity Does Not Require Functional Arf6- and Dynamin-dependent Endocytic PathwaysD⃞V⃞

Massol, Ramiro H., Larsen, Jakob E., Fujinaga, Yukako, Lencer, Wayne I., Kirchhausen, Tomas

Cholera toxin (CT) and related AB5 toxins bind to glycolipids at the plasma membrane and are then transported in a retrograde manner, first to the Golgi and then to the endoplasmic reticulum (ER). In...

Retrograde transport of cholera toxin from the plasma membrane to the endoplasmic reticulum requires the trans-Golgi network but not the Golgi apparatus in Exo2-treated cells

Feng, Yan, Jadhav, Ashutosh P, Rodighiero, Chiara, Fujinaga, Yukako, Kirchhausen, Tomas, Lencer, Wayne I

Cholera toxin (CT) follows a glycolipid-dependent entry pathway from the plasma membrane through the trans-Golgi network (TGN) to the endoplasmic reticulum (ER) where it is retro-translocated into...

The genome sequence of Clostridium botulinum type C neurotoxin-converting phage and the molecular mechanisms of unstable lysogeny

Sakaguchi, Yoshihiko, Hayashi, Tetsuya, Kurokawa, Ken, Nakayama, Keisuke, Oshima, Kenshiro, Fujinaga, Yukako, ...

Botulinum neurotoxins (BoNTXs) produced by Clostridium botulinum are among the most poisonous substances known. Of the seven types of BoNTXs, genes for type C1 and D toxins (BoNTX/C1 and D) are...