Asada, Kiyozo, Sugimoto, Kazunori, Oka, Atsuhiro, Takanami, Mituru, Hirota, Yukinori
The replication origin region of the Escherichia coli K-12 chromosome has been cloned, and a region of 245 base-pairs has been shown to contain all the information for autonomous replication (defined...
Nucleotide sequence of the asnA gene coding for asparagine synthetase of E.coli K-12 (1981)
Nakamura, Masataka, Yamada, Masao, Hirota, Yukinori, Sugimoto, Kazunori, Oka, Atsuhiro, Takanami, Mituru
We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin,...
Noguchi, Shigeru, Yamaizumi, Ziro, Ohgi, Tadaaki, Goto, Toshio, Nishimura, Yukinobu, Hirota, Yukinori, ...
One of the E. coli mutants selected for deficiency of modified nucleoside Q was found to contain an analogue of Q and normal guanosine in place of Q. The analogue of Q, designated as preQo, was...
Genetic approach to DNA replication and its control in Escherichia coli / by Y. Hirota and others
Separata de: Federation Proceedings, 1972, 31 (5) pp. 1422- 1427
Hirota, Yukinori, Mordoh, José
Separata de: J. Mol. Biol., 1970, 53, 369-387
Ricard, Matthieu, Hirota, Yukinori
Thermosensitive fts mutants of Escherichia coli belonging to seven previously identified genetic classes (ftsA to ftsG) were studied from a physiological standpoint. These mutants immediately stopped...
EFFECT OF DRUG-RESISTANCE FACTOR R ON THE F PROPERTIES OF ESCHERICHIA COLI
Hirota, Yukinori, Nishimura, Yukinobu, Ørskov, Frits, Ørskov, Ida
Hirota, Yukinori (Osaka University, Osaka, Japan), Yukinobu Nishimura, Frits Ørskov, and Ida Ørskov. Effect of drug-resistance factor R on the F properties of Escherichia coli. J. Bacteriol....
CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F′ INCORPORATING THEM
Ishibashi, Masahide, Sugino, Yoshinobu, Hirota, Yukinori
Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F′ incorporating them. J....
CONJUGAL FERTILITY ASSOCIATED WITH RESISTANCE FACTOR R IN ESCHERICHIA COLI
Sugino, Yoshinobu, Hirota, Yukinori
Sugino, Yoshinobu (Osaka University, Osaka, Japan) and Yukinori Hirota. Conjugal fertility associated with resistance factor R in Escherichia coli. J. Bacteriol. 84:902–910. 1962.—The...
Mordoh, José, Hirota, Yukinori, Jacob, François
Double mutants of Escherichia coli were constructed by combining DNA thermosensitive mutations affecting either DNA initiation (DnaA) or elongation (DnaB) with the Pol A1 mutation, which abolishes...
Impaired Transduction of R213 and Its Recovery by a Homologous Resident R Factor
Yoshikawa, Masanosuke, Hirota, Yukinori
Transduction by Plkc of drug-resistance markers of the factor R213 was shown to occur at an exceptionally low frequency (at less than 10−8 of the input phage), and they could not be transduced by...
Gene Recombination and Segregation of Resistance Factor R in Escherichia coli
Hashimoto, Hajime, Hirota, Yukinori
Hashimoto, Hajime (Osaka University, Osaka, Japan), and Yukinori Hirota. Gene recombination and segregation of resistance factor R in Escherichia coli. J. Bacteriol. 91:51–62. 1966.—Independent...
Hirota, Yukinori, Fujii, Toshio, Nishimura, Yukinobu
Hirota, Yukinori (University of Osaka, Osaka, Japan), Toshio Fujii, and Yukinobu Nishimura. Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in...
Lindahl, Gunnar, Hirota, Yukinori, Jacob, François
The temperature-sensitive mutant CRT46 of Escherichia coli K12 is unable to initiate new rounds of DNA replication at 42°C. Mutants of bacteriophage P2 have been isolated, which, in the prophage...
Gefter, Malcolm L., Hirota, Yukinori, Kornberg, Thomas, Wechsler, James A., Barnoux, C.
A series of double mutants carrying one of the thermosensitive mutations for DNA synthesis (dnaA, B, C, D, E, F, and G) and the polA1 mutation of DeLucia and Cairns, were constructed. Enzyme...
A Mutant of Escherichia coli Defective in DNA Polymerase II Activity
Hirota, Yukinori, Gefter, Malcolm, Mindich, Leonard
A mutant of E. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to...
Ricard, Matthieu, Hirota, Yukinori
Thermosensitive fts mutants of Escherichia coli belonging to seven previously identified genetic classes (ftsA to ftsG) were studied from a physiological standpoint. These mutants immediately stopped...
EFFECT OF DRUG-RESISTANCE FACTOR R ON THE F PROPERTIES OF ESCHERICHIA COLI
Hirota, Yukinori, Nishimura, Yukinobu, Ørskov, Frits, Ørskov, Ida
Hirota, Yukinori (Osaka University, Osaka, Japan), Yukinobu Nishimura, Frits Ørskov, and Ida Ørskov. Effect of drug-resistance factor R on the F properties of Escherichia coli. J. Bacteriol....
CHROMOSOMAL LOCATION OF THYMINE AND ARGININE GENES IN ESCHERICHIA COLI AND AN F′ INCORPORATING THEM
Ishibashi, Masahide, Sugino, Yoshinobu, Hirota, Yukinori
Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F′ incorporating them. J....
CONJUGAL FERTILITY ASSOCIATED WITH RESISTANCE FACTOR R IN ESCHERICHIA COLI
Sugino, Yoshinobu, Hirota, Yukinori
Sugino, Yoshinobu (Osaka University, Osaka, Japan) and Yukinori Hirota. Conjugal fertility associated with resistance factor R in Escherichia coli. J. Bacteriol. 84:902–910. 1962.—The...
Mordoh, José, Hirota, Yukinori, Jacob, François
Double mutants of Escherichia coli were constructed by combining DNA thermosensitive mutations affecting either DNA initiation (DnaA) or elongation (DnaB) with the Pol A1 mutation, which abolishes...
Impaired Transduction of R213 and Its Recovery by a Homologous Resident R Factor
Yoshikawa, Masanosuke, Hirota, Yukinori
Transduction by Plkc of drug-resistance markers of the factor R213 was shown to occur at an exceptionally low frequency (at less than 10−8 of the input phage), and they could not be transduced by...
Gene Recombination and Segregation of Resistance Factor R in Escherichia coli
Hashimoto, Hajime, Hirota, Yukinori
Hashimoto, Hajime (Osaka University, Osaka, Japan), and Yukinori Hirota. Gene recombination and segregation of resistance factor R in Escherichia coli. J. Bacteriol. 91:51–62. 1966.—Independent...
Hirota, Yukinori, Fujii, Toshio, Nishimura, Yukinobu
Hirota, Yukinori (University of Osaka, Osaka, Japan), Toshio Fujii, and Yukinobu Nishimura. Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in...
Lindahl, Gunnar, Hirota, Yukinori, Jacob, François
The temperature-sensitive mutant CRT46 of Escherichia coli K12 is unable to initiate new rounds of DNA replication at 42°C. Mutants of bacteriophage P2 have been isolated, which, in the prophage...
Gefter, Malcolm L., Hirota, Yukinori, Kornberg, Thomas, Wechsler, James A., Barnoux, C.
A series of double mutants carrying one of the thermosensitive mutations for DNA synthesis (dnaA, B, C, D, E, F, and G) and the polA1 mutation of DeLucia and Cairns, were constructed. Enzyme...
A Mutant of Escherichia coli Defective in DNA Polymerase II Activity
Hirota, Yukinori, Gefter, Malcolm, Mindich, Leonard
A mutant of E. coli defective in DNA polymerase II activity was isolated. Extracts had about 0.1% of the normal activity of the DNA polymerase. The effect of the mutation on the ability of cells to...