Fritz, Joëlle V, Didier, Pascal, Clamme, Jean-Pierre, Schaub, Emmanuel, Muriaux, Delphine, Cabanne, Charlotte, ...
Abstract Background The human immunodeficiency virus type 1 (HIV-1) encodes several regulatory proteins, notably Vpr which influences the survival of the infected cells by causing a G2/M arrest and...
Egelé, Caroline, Barbier, Pascale, Didier, Pascal, Piémont, Etienne, Allegro, Diane, Chaloin, Olivier, ...
Abstract Background During HIV-1 infection, the Tat protein plays a key role by transactivating the transcription of the HIV-1 proviral DNA. In addition, Tat induces apoptosis of non-infected T...
6thInternational Symposium on Retroviral Nucleocapsid (2008)
Berkhout, Ben, Gorelick, Robert, Summers, Michael F, Mély, Yves, Darlix, Jean-Luc
Abstract Retroviruses and LTR-retrotransposons are widespread in all living organisms and, in some instances such as for HIV, can be a serious threat to the human health. The retroviral nucleocapsid...
Klymchenko, Andrey S, Mély, Yves, Demchenko, Alexander P, Duportail, Guy
The penetration of water into the hydrophobic interior leads to polarity and hydration profiles across lipid membranes which are fundamental in the maintenance of membrane architecture as well as in...
Klymchenko, Andrey S, Duportail, Guy, Demchenko, Alexander P, Mély, Yves
A remarkable heterogeneity is often observed in the spectroscopic properties of environment-sensitive fluorescence probes in phospholipid bilayers. To explain its origin, we provided a detailed...
Llères, David, Weibel, Jean-Marc, Heissler, Denis, Zuber, Guy, Duportail, Guy, Mély, Yves
BACKGROUND: Control of the structure and physicochemical properties of DNA complexed with nonviral vectors is essential for efficient biodistribution and gene delivery to cells. Cationic liposomes...
Ultrasensitive two-color fluorescence probes for dipole potential in phospholipid membranes. (2007)
Klymchenko, Andrey S, Duportail, Guy, Mély, Yves, Demchenko, Alexander P
The principle of electrochromic modulation of excited-state intramolecular proton-transfer reaction was applied for the design of fluorescence probes with high two-color sensitivity to dipole...
Krishnamoorthy, G., Duportail, Guy, Mély, Yves
Information on the structure and dynamics of condensed forms of DNA is important in understanding both natural situations such as DNA packaging and artificial systems such as gene delivery complexes....
Klymchenko, Andrey S, Duportail, Guy, Ozturk, Turan, Pivovarenko, Vasyl G, Mély, Yves, Demchenko, Alexander P
3-hydroxyflavone (3-HF) derivatives are very attractive fluorescence sensors due to their ability to respond to small changes in their microenvironment via a dramatic alteration of the relative...
Yushchenko, Dmytro A, Shvadchak, Volodymyr V, Bilokin', Mykhailo D, Klymchenko, Andrey S, Duportail, Guy, Mély, Yves, ...
A representative of a new class of dyes with dual fluorescence due to an excited state intramolecular proton transfer (ESIPT) reaction, namely 1-methyl-2-(4-methoxy)phenyl-3-hydroxy-4(1H)-quinolone...
Ercelen, Sebnem, Zhang, Xin, Duportail, Guy, Grandfils, Christian, Desbrières, Jacques, Karaeva, Svetlana, ...
Low molecular weight chitosans grafted with N-/2(3)-(dodec-2-enyl)succinoyl groups (HM-LMW-chitosans) with a mean molecular mass of 5 kDa, a degree of acetylation of 3% and a degree of tetradecenoyl...
M'Baye, Gora, Shynkar, Vasyl V, Klymchenko, Andrey S, Mély, Yves, Duportail, Guy
We previously applied the electrochromic modulation of excited-state intramolecular proton-transfer (ESIPT) reaction for the design of novel 3-hydroxyflavone (3-HF) derivatives as fluorescent probes...
Two-color fluorescent probes for imaging the dipole potential of cell plasma membranes. (2007)
Shynkar, Vasyl V, Klymchenko, Andrey S, Duportail, Guy, Demchenko, Alexander P, Mély, Yves
The dipole potential (Psi(d)) constitutes a large and functionally important part of the electrostatic potential of cell plasma membranes. However, its direct measurement is not possible. Herein, new...
M'Baye, Gora, Klymchenko, Andrey S, Yushchenko, Dmytro A, Shvadchak, Volodymyr V, Ozturk, Turan, Mély, Yves, ...
4'-(Dialkylamino)-3-hydroxyflavones are characterized by an excited-state proton transfer reaction between two tautomeric excited states, which results in two emission bands well separated on the...
Optimization and antiviral analysis of peptide ligands for the HIV-1 packaging signal PSI (2006)
Dietz, Julia, Pustowka, Anette, Kaur, Ajit, Koch, Joachim, Mensch, Sarah, Stein, Stefan, ...
No abstract available.
Molecular beacons are hairpin-shaped, single-stranded oligonucleotides constituting sensitive fluorescent DNA probes widely used to report the presence of specific nucleic acids. In its closed form...
DNA condensation by the nucleocapsid protein of HIV-1: a mechanism ensuring DNA protection
Krishnamoorthy, G., Roques, Bernard, Darlix, Jean-Luc, Mély, Yves
The nucleocapsid (NC) protein NCp7 of the immunodeficiency virus type 1 is a small basic protein with two zinc finger motifs. NCp7 has key roles in virus replication and structure, which rely on its...
Ultrasensitive two-color fluorescence probes for dipole potential in phospholipid membranes
Klymchenko, Andrey S., Duportail, Guy, Mély, Yves, Demchenko, Alexander P.
The principle of electrochromic modulation of excited-state intramolecular proton-transfer reaction was applied for the design of fluorescence probes with high two-color sensitivity to dipole...
Gaied, Nouha Ben, Glasser, Nicole, Ramalanjaona, Nick, Beltz, Hervé, Wolff, Philippe, Marquet, Roland, ...
We report here the synthesis and the spectroscopic characterization of 8-vinyl-deoxyadenosine (8vdA), a new fluorescent analog of deoxyadenosine. 8vdA was found to absorb and emit in the same...
Bourbigot, Sarah, Beltz, Hervé, Denis, Jérôme, Morellet, Nelly, Roques, Bernard P., Mély, Yves, ...
HIV-1 Vpr is a highly conserved accessory protein that is involved in many functions of the virus life cycle. Vpr facilitates the entry of the HIV pre-integration complex through the nuclear pore,...
Bousquet, Jean-Alain, Duranton, Jérôme, Mély, Yves, Bieth, Joseph G
The CD spectrum of porcine pancreatic elastase in complex with alpha1-proteinase inhibitor (alpha1-PI) was calculated by subtracting the CD spectrum of the proteolytically cleaved inhibitor from that...
Bernacchi, Serena, Piémont, Etienne, Potier, Noelle, Dorsselaer, Alain Van, Mély, Yves
In this study, we investigated the absorbance and fluorescence properties of cTAR, the complementary DNA sequence of the transactivation response element of the HIV-1 genome, doubly end-labeled by...
Clamme, Jean Pierre, Azoulay, Joel, Mély, Yves
Polyethylenimines (PEI) constitute efficient nonviral vectors for gene transfer. However, because free PEI shows some cytotoxicity and because intracellular dissociation of PEI/DNA complexes seems to...
Bimodal Distribution and Fluorescence Response of Environment-Sensitive Probes in Lipid Bilayers
Klymchenko, Andrey S., Duportail, Guy, Demchenko, Alexander P., Mély, Yves
A remarkable heterogeneity is often observed in the spectroscopic properties of environment-sensitive fluorescence probes in phospholipid bilayers. To explain its origin, we provided a detailed...
Molecular beacons are hairpin-shaped, single-stranded oligonucleotides constituting sensitive fluorescent DNA probes widely used to report the presence of specific nucleic acids. In its closed form...
DNA condensation by the nucleocapsid protein of HIV-1: a mechanism ensuring DNA protection
Krishnamoorthy, G., Roques, Bernard, Darlix, Jean-Luc, Mély, Yves
The nucleocapsid (NC) protein NCp7 of the immunodeficiency virus type 1 is a small basic protein with two zinc finger motifs. NCp7 has key roles in virus replication and structure, which rely on its...
Ultrasensitive two-color fluorescence probes for dipole potential in phospholipid membranes
Klymchenko, Andrey S., Duportail, Guy, Mély, Yves, Demchenko, Alexander P.
The principle of electrochromic modulation of excited-state intramolecular proton-transfer reaction was applied for the design of fluorescence probes with high two-color sensitivity to dipole...
Gaied, Nouha Ben, Glasser, Nicole, Ramalanjaona, Nick, Beltz, Hervé, Wolff, Philippe, Marquet, Roland, ...
We report here the synthesis and the spectroscopic characterization of 8-vinyl-deoxyadenosine (8vdA), a new fluorescent analog of deoxyadenosine. 8vdA was found to absorb and emit in the same...
Bourbigot, Sarah, Beltz, Hervé, Denis, Jérôme, Morellet, Nelly, Roques, Bernard P., Mély, Yves, ...
HIV-1 Vpr is a highly conserved accessory protein that is involved in many functions of the virus life cycle. Vpr facilitates the entry of the HIV pre-integration complex through the nuclear pore,...
Bousquet, Jean-Alain, Duranton, Jérôme, Mély, Yves, Bieth, Joseph G
The CD spectrum of porcine pancreatic elastase in complex with alpha1-proteinase inhibitor (alpha1-PI) was calculated by subtracting the CD spectrum of the proteolytically cleaved inhibitor from that...
Bernacchi, Serena, Piémont, Etienne, Potier, Noelle, Dorsselaer, Alain Van, Mély, Yves
In this study, we investigated the absorbance and fluorescence properties of cTAR, the complementary DNA sequence of the transactivation response element of the HIV-1 genome, doubly end-labeled by...
Clamme, Jean Pierre, Azoulay, Joel, Mély, Yves
Polyethylenimines (PEI) constitute efficient nonviral vectors for gene transfer. However, because free PEI shows some cytotoxicity and because intracellular dissociation of PEI/DNA complexes seems to...
Bimodal Distribution and Fluorescence Response of Environment-Sensitive Probes in Lipid Bilayers
Klymchenko, Andrey S., Duportail, Guy, Demchenko, Alexander P., Mély, Yves
A remarkable heterogeneity is often observed in the spectroscopic properties of environment-sensitive fluorescence probes in phospholipid bilayers. To explain its origin, we provided a detailed...
Optimization and antiviral analysis of peptide ligands for the HIV-1 packaging signal PSI
Dietz, Julia, Pustowka, Anette, Kaur, Ajit, Koch, Joachim, Mensch, Sarah, Stein, Stefan, ...
6th International Symposium on Retroviral Nucleocapsid
Berkhout, Ben, Gorelick, Robert, Summers, Michael F, Mély, Yves, Darlix, Jean-Luc
Retroviruses and LTR-retrotransposons are widespread in all living organisms and, in some instances such as for HIV, can be a serious threat to the human health. The retroviral nucleocapsid is the...
Bombarda, Elisa, Grell, Ernst, Roques, Bernard P., Mély, Yves
HIV-1 nucleocapsid protein, NCp7, contains two highly conserved CCHC zinc fingers. Binding of Zn2+ drives NCp7 from an unfolded to a highly folded structure that is critical for its functions. Using...
The HIV-1 transcriptional activator Tat has potent nucleic acid chaperoning activities in vitro
Kuciak, Monika, Gabus, Caroline, Ivanyi-Nagy, Roland, Semrad, Katharina, Storchak, Roman, Chaloin, Olivier, ...
The human immunodeficiency virus type 1 (HIV-1) is a primate lentivirus that causes the acquired immunodeficiency syndrome (AIDS). In addition to the virion structural proteins and enzyme precursors,...