Zhi-Ming Zheng

Oncogenic HPV infection interrupts the expression of tumor-suppressive miR-34a through viral oncoprotein E6 (2009)

Wang, Xiaohong, Wang, Hsu-Kun, McCoy, J. Philip, Banerjee, Nilam S., Rader, Janet S., Broker, Thomas R., ...

MicroRNAs (miRNA) play pivotal roles in controlling cell proliferation and differentiation. Aberrant miRNA expression in human is becoming recognized as a new molecular mechanism of carcinogenesis....

Suppression by delta-9-Tetrahydrocannabinol of tumor necrosis factor maturation and secretion and protein tyrosine phosphorylation in mouse resident peritoneal macrophages /--by Zhi-Ming Zheng. (1994)

Zheng, Zhi-Ming.

Thesis (Ph. D.)--University of South Florida, 1994.

Utilization of the Bovine Papillomavirus Type 1 Late-Stage-Specific Nucleotide 3605 3′ Splice Site Is Modulated by a Novel Exonic Bipartite Regulator but Not by an Intronic Purine-Rich Element

Zheng, Zhi-Ming, Reid, Eric S., Baker, Carl C.

Bovine papillomavirus type 1 (BPV-1) late gene expression is regulated at both transcriptional and posttranscriptional levels. Maturation of the capsid protein (L1) pre-mRNA requires a switch in 3′...

Optimization of a Weak 3′ Splice Site Counteracts the Function of a Bovine Papillomavirus Type 1 Exonic Splicing Suppressor In Vitro and In Vivo

Zheng, Zhi-Ming, Quintero, Jesse, Reid, Eric S., Gocke, Christian, Baker, Carl C.

Alternative splicing is a critical component of the early to late switch in papillomavirus gene expression. In bovine papillomavirus type 1 (BPV-1), a switch in 3′ splice site utilization from an...

Exonic Splicing Enhancer-Dependent Selection of the Bovine Papillomavirus Type 1 Nucleotide 3225 3′ Splice Site Can Be Rescued in a Cell Lacking Splicing Factor ASF/SF2 through Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway

Liu, Xuefeng, Mayeda, Akila, Tao, Mingfang, Zheng, Zhi-Ming

Bovine papillomavirus type 1 (BPV-1) late pre-mRNAs are spliced in keratinocytes in a differentiation-specific manner: the late leader 5′ splice site alternatively splices to a proximal 3′ splice...

Signals That Dictate Nuclear Localization of Human Papillomavirus Type 16 Oncoprotein E6 in Living Cells

Tao, Mingfang, Kruhlak, Michael, Xia, Shuhua, Androphy, Elliot, Zheng, Zhi-Ming

Human papillomavirus (HPV) type 16 E6 (16E6) is an oncogenic, multifunctional nuclear protein that induces p53 degradation and perturbs normal cell cycle control, leading to immortalization and...

Requirement of a 12-Base-Pair TATT-Containing Sequence and Viral Lytic DNA Replication in Activation of the Kaposi's Sarcoma-Associated Herpesvirus K8.1 Late Promoter

Tang, Shuang, Yamanegi, Koji, Zheng, Zhi-Ming

Kaposi's sarcoma-associated herpesvirus (KSHV) K8.1 late promoter consists of a minimal 24-bp sequence, with a TATA-like, 12-bp promoter core, AATATTAAAGGG, and is active on a reporter only in...

Kaposi's Sarcoma-Associated Herpesvirus K8β Is Derived from a Spliced Intermediate of K8 Pre-mRNA and Antagonizes K8α (K-bZIP) To Induce p21 and p53 and Blocks K8α-CDK2 Interaction

Yamanegi, Koji, Tang, Shuang, Zheng, Zhi-Ming

Kaposi's sarcoma-associated herpesvirus (KSHV) is a lymphotropic DNA tumor virus that induces Kaposi's sarcoma and AIDS-related primary effusion lymphoma. KSHV open reading frame 50 and K8 genes in...

Could Human Papillomaviruses Be Spread through Blood?

Bodaghi, Sohrab, Wood, Lauren V., Roby, Gregg, Ryder, Celia, Steinberg, Seth M., Zheng, Zhi-Ming

The human papillomaviruses (HPVs) are epitheliotropic viruses that require the environment of a differentiating squamous epithelium for their life cycle. HPV infection through abrasion of the skin or...

Development of resistance to RNAi in mammalian cells

Zheng, Zhi-Ming, Tang, Shuang, Tao, Mingfang

The discovery of RNA interference (RNAi) in C. elegans and in plants has revolutionized current approaches to biology and medicine. RNAi silences genes in a sequence-specific manner through the...

PAPILLOMAVIRUS GENOME STRUCTURE, EXPRESSION, AND POST-TRANSCRIPTIONAL REGULATION

Zheng, Zhi-Ming, Baker, Carl C.

Papillomaviruses are a group of small non-enveloped DNA tumor viruses whose infection usually causes benign epithelial lesions (warts). Certain types of HPVs, such as HPV-16, HPV-18, and HPV-31, have...

The E7 Oncoprotein Is Translated from Spliced E6*I Transcripts in High-Risk Human Papillomavirus Type 16- or Type 18-Positive Cervical Cancer Cell Lines via Translation Reinitiation

Tang, Shuang, Tao, Mingfang, McCoy, J. Philip, Zheng, Zhi-Ming

High-risk human papillomaviruses (HPVs) encode two viral oncoproteins, E6 and E7, from a single bicistronic pre-mRNA containing three exons and two introns. Retention of intron 1 in the E6 coding...

Genetic Organization and Hypoxic Activation of the Kaposi's Sarcoma-Associated Herpesvirus ORF34-37 Gene Cluster†

Haque, Muzammel, Wang, Victoria, Davis, David A., Zheng, Zhi-Ming, Yarchoan, Robert

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent for Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). We previously reported that hypoxia activates KSHV lytic...

Utilization of the Bovine Papillomavirus Type 1 Late-Stage-Specific Nucleotide 3605 3′ Splice Site Is Modulated by a Novel Exonic Bipartite Regulator but Not by an Intronic Purine-Rich Element

Zheng, Zhi-Ming, Reid, Eric S., Baker, Carl C.

Bovine papillomavirus type 1 (BPV-1) late gene expression is regulated at both transcriptional and posttranscriptional levels. Maturation of the capsid protein (L1) pre-mRNA requires a switch in 3′...

Optimization of a Weak 3′ Splice Site Counteracts the Function of a Bovine Papillomavirus Type 1 Exonic Splicing Suppressor In Vitro and In Vivo

Zheng, Zhi-Ming, Quintero, Jesse, Reid, Eric S., Gocke, Christian, Baker, Carl C.

Alternative splicing is a critical component of the early to late switch in papillomavirus gene expression. In bovine papillomavirus type 1 (BPV-1), a switch in 3′ splice site utilization from an...

Exonic Splicing Enhancer-Dependent Selection of the Bovine Papillomavirus Type 1 Nucleotide 3225 3′ Splice Site Can Be Rescued in a Cell Lacking Splicing Factor ASF/SF2 through Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway

Liu, Xuefeng, Mayeda, Akila, Tao, Mingfang, Zheng, Zhi-Ming

Bovine papillomavirus type 1 (BPV-1) late pre-mRNAs are spliced in keratinocytes in a differentiation-specific manner: the late leader 5′ splice site alternatively splices to a proximal 3′ splice...

Signals That Dictate Nuclear Localization of Human Papillomavirus Type 16 Oncoprotein E6 in Living Cells

Tao, Mingfang, Kruhlak, Michael, Xia, Shuhua, Androphy, Elliot, Zheng, Zhi-Ming

Human papillomavirus (HPV) type 16 E6 (16E6) is an oncogenic, multifunctional nuclear protein that induces p53 degradation and perturbs normal cell cycle control, leading to immortalization and...

Requirement of a 12-Base-Pair TATT-Containing Sequence and Viral Lytic DNA Replication in Activation of the Kaposi's Sarcoma-Associated Herpesvirus K8.1 Late Promoter

Tang, Shuang, Yamanegi, Koji, Zheng, Zhi-Ming

Kaposi's sarcoma-associated herpesvirus (KSHV) K8.1 late promoter consists of a minimal 24-bp sequence, with a TATA-like, 12-bp promoter core, AATATTAAAGGG, and is active on a reporter only in...

Kaposi's Sarcoma-Associated Herpesvirus K8β Is Derived from a Spliced Intermediate of K8 Pre-mRNA and Antagonizes K8α (K-bZIP) To Induce p21 and p53 and Blocks K8α-CDK2 Interaction

Yamanegi, Koji, Tang, Shuang, Zheng, Zhi-Ming

Kaposi's sarcoma-associated herpesvirus (KSHV) is a lymphotropic DNA tumor virus that induces Kaposi's sarcoma and AIDS-related primary effusion lymphoma. KSHV open reading frame 50 and K8 genes in...

Could Human Papillomaviruses Be Spread through Blood?

Bodaghi, Sohrab, Wood, Lauren V., Roby, Gregg, Ryder, Celia, Steinberg, Seth M., Zheng, Zhi-Ming

The human papillomaviruses (HPVs) are epitheliotropic viruses that require the environment of a differentiating squamous epithelium for their life cycle. HPV infection through abrasion of the skin or...

The E7 Oncoprotein Is Translated from Spliced E6*I Transcripts in High-Risk Human Papillomavirus Type 16- or Type 18-Positive Cervical Cancer Cell Lines via Translation Reinitiation

Tang, Shuang, Tao, Mingfang, McCoy, J. Philip, Zheng, Zhi-Ming

High-risk human papillomaviruses (HPVs) encode two viral oncoproteins, E6 and E7, from a single bicistronic pre-mRNA containing three exons and two introns. Retention of intron 1 in the E6 coding...

Genetic Organization and Hypoxic Activation of the Kaposi's Sarcoma-Associated Herpesvirus ORF34-37 Gene Cluster†

Haque, Muzammel, Wang, Victoria, Davis, David A., Zheng, Zhi-Ming, Yarchoan, Robert

Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent for Kaposi's sarcoma (KS) and primary effusion lymphoma (PEL). We previously reported that hypoxia activates KSHV lytic...

Gene Structure and Expression of Kaposi's Sarcoma-Associated Herpesvirus ORF56, ORF57, ORF58, and ORF59▿

Majerciak, Vladimir, Yamanegi, Koji, Zheng, Zhi-Ming

Though similar to those of herpesvirus saimiri and Epstein-Barr virus (EBV), the Kaposi's sarcoma-associated herpesvirus (KSHV) genome features more splice genes and encodes many genes with...

Targeted Disruption of Kaposi's Sarcoma-Associated Herpesvirus ORF57 in the Viral Genome Is Detrimental for the Expression of ORF59, K8α, and K8.1 and the Production of Infectious Virus▿

Majerciak, Vladimir, Pripuzova, Natalia, McCoy, J. Philip, Gao, Shou-Jiang, Zheng, Zhi-Ming

Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 regulates viral gene expression at the posttranscriptional level during viral lytic infection. To study its function in the context of the viral...

Kaposi's Sarcoma-Associated Herpesvirus ORF57 Functions as a Viral Splicing Factor and Promotes Expression of Intron-Containing Viral Lytic Genes in Spliceosome-Mediated RNA Splicing▿

Majerciak, Vladimir, Yamanegi, Koji, Allemand, Eric, Kruhlak, Michael, Krainer, Adrian R., Zheng, Zhi-Ming

Kaposi's sarcoma-associated herpesvirus (KSHV) ORF57 facilitates the expression of both intronless viral ORF59 genes and intron-containing viral K8 and K8.1 genes (V. Majerciak, N. Pripuzova, J. P....

Aberrant Expression of Oncogenic and Tumor-Suppressive MicroRNAs in Cervical Cancer Is Required for Cancer Cell Growth

Wang, Xiaohong, Tang, Shuang, Le, Shu-Yun, Lu, Robert, Rader, Janet S., Meyers, Craig, ...

MicroRNAs (miRNAs) play important roles in cancer development. By cloning and sequencing of a HPV16+ CaSki cell small RNA library, we isolated 174 miRNAs (including the novel miR-193c) which could be...

Oncogenic HPV infection interrupts the expression of tumor-suppressive miR-34a through viral oncoprotein E6

Wang, Xiaohong, Wang, Hsu-Kun, McCoy, J. Philip, Banerjee, Nilam S., Rader, Janet S., Broker, Thomas R., ...

MicroRNAs (miRNA) play pivotal roles in controlling cell proliferation and differentiation. Aberrant miRNA expression in human is becoming recognized as a new molecular mechanism of carcinogenesis....

Control of the Papillomavirus Early-to-Late Switch by Differentially Expressed SRp20▿ †

Jia, Rong, Liu, Xuefeng, Tao, Mingfang, Kruhlak, Michael, Guo, Ming, Meyers, Craig, ...

The viral early-to-late switch of papillomavirus infection is tightly linked to keratinocyte differentiation and is mediated in part by alternative mRNA splicing. Here, we report that SRp20, a...