Zhiyong Ding

Activities of virE1 and the VirE1 Secretion Chaperone in Export of the Multifunctional VirE2 Effector via an Agrobacterium Type IV Secretion Pathway

Zhao, Zhenming, Sagulenko, Evgeniy, Ding, Zhiyong, Christie, Peter J.

Agrobacterium tumefaciens uses a type IV secretion system to deliver oncogenic nucleoprotein particles and effector proteins, such as the multifunctional VirE2 protein, to plant cells. In this study,...

A Novel Cytology-Based, Two-Hybrid Screen for Bacteria Applied to Protein-Protein Interaction Studies of a Type IV Secretion System

Ding, Zhiyong, Zhao, Zhenming, Jakubowski, Simon J., Krishnamohan, Atmakuri, Margolin, William, Christie, Peter J.

DivIVA of Bacillus subtilis and FtsZ of Escherichia coli were used to target heterologous protein complexes to cell division sites of E. coli and Agrobacterium tumefaciens. DivIVA and FtsZ that were...

Agrobacterium tumefaciens Twin-Arginine-Dependent Translocation Is Important for Virulence, Flagellation, and Chemotaxis but Not Type IV Secretion

Ding, Zhiyong, Christie, Peter J.

This study characterized the contribution of the twin-arginine translocation (TAT) pathway to growth, motility, and virulence of the phytopathogen Agrobacterium tumefaciens. In contrast to wild-type...

Activities of virE1 and the VirE1 Secretion Chaperone in Export of the Multifunctional VirE2 Effector via an Agrobacterium Type IV Secretion Pathway

Zhao, Zhenming, Sagulenko, Evgeniy, Ding, Zhiyong, Christie, Peter J.

Agrobacterium tumefaciens uses a type IV secretion system to deliver oncogenic nucleoprotein particles and effector proteins, such as the multifunctional VirE2 protein, to plant cells. In this study,...

A Novel Cytology-Based, Two-Hybrid Screen for Bacteria Applied to Protein-Protein Interaction Studies of a Type IV Secretion System

Ding, Zhiyong, Zhao, Zhenming, Jakubowski, Simon J., Krishnamohan, Atmakuri, Margolin, William, Christie, Peter J.

DivIVA of Bacillus subtilis and FtsZ of Escherichia coli were used to target heterologous protein complexes to cell division sites of E. coli and Agrobacterium tumefaciens. DivIVA and FtsZ that were...

Agrobacterium tumefaciens Twin-Arginine-Dependent Translocation Is Important for Virulence, Flagellation, and Chemotaxis but Not Type IV Secretion

Ding, Zhiyong, Christie, Peter J.

This study characterized the contribution of the twin-arginine translocation (TAT) pathway to growth, motility, and virulence of the phytopathogen Agrobacterium tumefaciens. In contrast to wild-type...

A retrovirus-based protein complementation assay screen reveals functional AKT1-binding partners

Ding, Zhiyong, Liang, Jiyong, Lu, Yiling, Yu, Qinghua, Songyang, Zhou, Lin, Shiaw-Yih, ...

We developed a retrovirus-based protein-fragment complementation assay (RePCA) screen to identify protein–protein interactions in mammalian cells. In RePCA, bait protein is fused to one fragment of...